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Acknowledgments Research in the Beuning laboratory is supported by a New Faculty Award from the Camille Henry Dreyfus Foundation and a CAREER Award from the NSF (MCB- 0845033 to PJB). Such curves can be fitted using a second-order polynomial equation: SS0 14 aD þ bD2 ð8:2Þ where a and b are the fit parameters and solving for D when SS0 14 0:37 yields the D37 value.

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